RTS-8, Multi-channel Bioreactor with non-invasive real time biomass, pH and pO2measurement Description RTS-8 is a personal bioreactor that utilizes patented Reverse-Spin® technology that applies non-invasive, mechanically driven, low energy consumption, innovative type of agitation where cell suspension is mixed by the single-use falcon bioreactor tube rotation around its axis with a change of direction of rotation motion resulting in highly efficient mixing and oxygenation for aerobic cultivation. Combined with a nearinfrared optical system it is possible to register cell growth kinetics non-invasively in real time.
FEATURES
Parallel cultivation enables to save time and resources for bioprocess optimization Individually controlled bioreactor accelerates optimization process Possibility to cultivate microaerophilic and obligate anaerobic microorganisms (not strict anaerobic conditions) Reverse–Spin® mixing principle enables non-invasive biomass measurement in real time Near-infrared optical system makes it possible to register cell growth kinetics Free of charge software for storage, demonstration and analysis of data in real time Compact design with low profile and small footprint for personal application Temperature control for bioprocess applications Active cooling for rapid temperature control, e.g. for temperature fluctuation experiments Task profiling for process automatization Cloud data storage to remotely monitor the process of cultivation while at home or using a mobile phone
SOFTWARE FEATURES
Real–Time cell growth logging 3D graphical representation of OD or growth rate over time over unit Pause option Save/Load option Report option: PDF and Excel Connect up to 12 units (recommended) simultaneously to 1 computer Remote monitoring option (requires internet connection) Cycling/Profiling options User manual calibration possibility for most cells
TYPICAL APPLICATIONS
Fermentation real time growth kinetics Clone candidate screening Protein expression Temperature stress and fluctuation experiments Media screening and optimization Growth characterization Inhibition and toxicity tests Strain quality control
Specification Measurement range0-100 OD600 Measurement wavelength (λ)850 ± 15 nm Light sourceLaser Measurement periodicity per hour1-60 Temperature setting range+15°C ... +60°C Top control range point60°C Sample volume3–50 ml Speed control range50–2,700 rpm Max. number of units connected to the software12 DisplayLCD Minimum PC requirementsIntel/AMD Processor, 1 GB RAM, Windows XP*/Vista/7/8/8.1, 2.0 USB port Optimal PC requirementsIntel/AMD Processor, 3 GB RAM Windows XP*/Vista/7/8/8.1/10, USB 2.0 port
* not guaranteed because OS not supported by producer Dimensions (W×D×H)330 × 580 × 320 mm Weight15 kg Internal power supplyInput AC 100–240 V 50/60 Hz; Output DC 12 V ------ 02 sensor+ Range0-100% Accuracy± 0.01% O2 at 0.21%, ± 0.1% O2 at 20.9% Drift< 0.015% O2 per Day Temperature rangeup to 40°C ------- pH sensor+ RangepH 4.0 - pH 8.5 Accuracy± 0.05 pH at pH 7 with one-point calibration, ± 0.10 pH at pH 7 with precalibration Drift< 0.005 pH per day Temperature range pHup to 40°C
